Submit Manuscript  

Article Details


A Combination of Egg Yolk IgY and Phosvitin Inhibits the Growth of Enterotoxigenic Escherichia coli K88 and K99

[ Vol. 18 , Issue. 5 ]

Author(s):

Naiyana Gujral, Heejoo Yoo, Fatemeh Bamdad, Kwang Y. Lee, Joo-Won Suh and Hoon Sunwoo   Pages 400 - 409 ( 10 )

Abstract:


Background: Enterotoxigenic Escherichia coli (ETEC) is the main cause of fatal diarrhea in piglets during the first week of life and over the time of weaning. Pathogenesis of ETEC-causing diarrhea involves intestinal colonization mediated by fimbriae. Although, both IgY and egg yolk phosvitin (PV) possess antimicrobial activity, their combined activity has not been explored. A combination of IgY specific for ETEC and metal-chelating PV may show synergistic effect in reducing the growth of ETEC by inhibiting bacterial proliferation and stipulating protection against ETEC infection.

Objective: The goal of this study was to determine the effects of anti-ETEC IgY and PV on in vitro growth inhibition of ETEC strains possessing K88 and K99 fimbriae prevalent in the porcine population.

Methods: Anti-K88 and -K99 IgY antibodies were obtained from egg yolks of 23-week-old Single- Comb White Leghorn hens immunized with K88 and K99 fimbriae of ETEC, respectively, with high titres sustained over 6 to 8 weeks of the immunization period. Specific IgY, PV, and PV-hydrolysate from alcalase-hydrolysis under high hydrostatic pressure (PVH-Alc-HHP) alone or in combination, were used to treat ETEC K88 and K99 cultures at optimal concentrations of 100 μg/mL, 1 mg/mL, and 1 mg/mL, respectively, for 24 h.

Results: PVH-Alc-HHP demonstrated the highest degree of hydrolysis, 38.9%. Combined use of IgY and PVH-Alc-HHP showed the highest bactericidal effect resulting in ETEC K88 and K99 growth inhibition of 2.8 and 2.67 log CFU/mL, respectively.

Conclusion: Combined IgY-PVH effectively control ETEC, therefore holds a great potential for microbial control in veterinary pharmaceutical industry.

Keywords:

Antimicrobial, egg yolk IgY antibody, Escherichia coli K88, growth inhibition, high hydrostatic pressure, K99, phosvitin.

Affiliation:

Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 114 St-89 Ave., Edmonton, Alberta, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 114 St-89 Ave., Edmonton, Alberta, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 114 St-89 Ave., Edmonton, Alberta, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 114 St-89 Ave., Edmonton, Alberta, Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Gyeonggi, 449-728, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 114 St-89 Ave., Edmonton, Alberta

Graphical Abstract:



Read Full-Text article