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Water-in-Oil Microemulsions for Protein Delivery: Loading Optimization and Stability

[ Vol. 18 , Issue. 5 ]

Author(s):

Diego R. Perinelli, Marco Cespi, Stefania Pucciarelli, Silvia Vincenzetti, Luca Casettari, Jenny K.W. Lam, Serena Logrippo, Elisa Canala, Mahmoud E. Soliman, Giulia Bonacucina and Giovanni F. Palmieri   Pages 410 - 421 ( 12 )

Abstract:


Background: Microemulsions are attractive delivery systems for therapeutic proteins and peptides due to their ability to enhance bioavailability. Although different proteins and peptides have been successfully delivered through such ternary systems, no information can be found about protein loading and the formulation stability when such microemulsions are prepared with pharmaceuticallyapproved oils and surfactants. The aim of this work was to optimise a ternary system consisting of water/ ethyl oleate/Span® 80-Tween® 80 and to determine its protein loading capacity and stability, using bovine serum albumin (BSA) as a model of biomolecule.

Methods: The optimization was carried out using a Central Composite Design and all the prepared formulations were characterised through dynamic light scattering, rheology, optical and polarized microscopy. Subsequently, the maximum loading capacity was determined and the stability of the final microemulsion with the highest content of protein was followed over six months. To investigate the structural features of the protein, BSA was recovered from the microemulsion and analysed through fluorescence spectroscopy.

Results: After incorporation of the protein in the microemulsion, a decrease of its aqueous solubility was observed. However, the formulation remained stable over six months and the native-like state of the recovered protein was demonstrated by fluorescence spectroscopy

Conclusion: This study demonstrated the feasibility of preparing microemulsions with the highest content of protein and their long-term stability.

Keywords:

Design of experiments (DoE), formulation stability, protein loading, protein solubility, SDS-PAGE, ternary system.

Affiliation:

School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, School of Biosciences and Veterinary Medicine, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, School of Biosciences and Veterinary Medicine, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, Department of Biomolecular Sciences, School of Pharmacy, University of Urbino, Piazza del Rinascimento, 6, 61029 Urbino (PU), Department of Pharmacology & Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino (MC), School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC

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