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Molecular Imaging of Tumor Angiogenesis and Therapeutic Effects with Dual Bioluminescence

[ Vol. 18 , Issue. 5 ]

Author(s):

Ran Wang, Kaiyue Zhang, Hongyan Tao, Wei Du, Di Wang, Ziwei Huang, Manqian Zhou, Yang Xu, Yuebing Wang, Na Liu, Hui Wang and Zongjin Li   Pages 422 - 428 ( 7 )

Abstract:


Background: Angiogenesis is critical for the growth of tumor by supplying nutrients and oxygen that exacerbates the metastasis and progression of cancer. Noninvasive imaging of angiogenesis during the tumor therapeutic processes may provide novel opportunities for image-guided tumor management.

Objective: Here, we want to develop a mouse animal model for assessing cancer progression and angiogenesis in the same individuals by molecular imaging.

Methods: Breast cancer model was developed with mouse breast cancer cell line 4T1 carrying a reporter system encoding a triple fusion (TF) reporter gene consisting of renilla luciferase (Rluc), red fluorescent protein (RFP) and herpes simplex virus truncated thymidine kinase (HSV-ttk) in transgenic mice, which expressed firefly luciferase (Fluc) under the promoter of vascular endothelial growth factor receptor 2 (Vegfr2-luc). The mice were subsequently treated with ganciclovir (GCV) and the tumor angiogenesis was tracked by Fluc imaging and the growth status of tumor was monitored by imaging of Rluc simultaneously.

Conclusion: Overall, this traceable breast cancer model can simultaneously image the tumor growth and angiogenesis in single individual, which may facilitate a better understanding the mechanisms of angiogenesis in the progression and regression of tumor.

Keywords:

Angiogenesis, bioluminescence imaging (BLI), renilla luciferase (Rluc), firefly luciferase (Fluc), vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR).

Affiliation:

Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Nankai University School of Medicine, Tianjin 300071, Department of Radiation Oncology, Tianjin Union Medical Center, 190 Jieyuan Rd, Tianjin 300121, Nankai University School of Medicine, 94 Weijin Road, Tianjin 300071

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