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Immunomodulatory Effects of Bifidobacterium longum W11 Produced Exopolysaccharide on Cytokine Production

[ Vol. 18 , Issue. 11 ]

Author(s):

Rosanna Inturri*, Katia Mangano, Maria Santagati, Mariano Intrieri, Roberto Di Marco and Giovanna Blandino   Pages 883 - 889 ( 7 )

Abstract:


Background: Health benefits, including immune modulating capability, exerted by Bifidobacterium strains have been attributed to their exopolysaccharides (EPSs).

Objective: The effects of the purified EPS from B. longum W11 on cytokine production by peripheral blood mononuclear cells (PBMCs) alone or ConA-stimulated were investigated.

Method: The production of IFN-γ, IL-1β, IL-6 and IL-10 by PBMCs from healthy adult donors was analysed using purified EPS at two different concentrations (100 µg/mL and 200 µg/mL) and ConA, as an immunopotentiating marker. Moreover, the monosaccharide composition of the EPS from B. longum W11 was detected using HPLC analysis.

Results: The results demonstrated the ability of purified EPS to increase the production of the tested cytokines, except IL-10, in ConA-stimulated PBMCs. In not-stimulated-PBMCs, EPS increased the production of IL-6 (at 200 µg/mL) and IL-10 (at 100 µg/mL). The HPLC analysis showed the presence of main monomers, galactose and glucose (ratio 1:1 wt/wt), and small amount of rhamnose.

Conclusion: The results of this study demonstrate the ability of the EPS produced by B. longum W11 to interact in vitro with the human PBMCs, showing an immune-regulatory profile alone and an immune stimulatory profile in ConA-stimulated PBMCs. This suggests putative applications for the EPS from B. longum W11 in different pathological conditions.

Keywords:

Bifidobacterium longum, probiotic, exopolysaccharide, chromatographic analysis, immunomodulatory effects, cytokine production, PBMC, HPLC.

Affiliation:

Department of Biomedical and Biotechnological Sciences, Section of Microbiology, University of Catania, Via Santa Sofia 97, 95123 Catania (CT), Department of Biomedical and Biotechnological Sciences, Section of Oncologic, Clinic and General Pathology, University of Catania, Via Santa Sofia 97, 95123 Catania (CT), Department of Biomedical and Biotechnological Sciences, Section of Microbiology, University of Catania, Via Santa Sofia 97, 95123 Catania (CT), Department of Medicine and Health Sciences , Department of Medicine and Health Sciences , Department of Biomedical and Biotechnological Sciences, Section of Microbiology, University of Catania, Via Santa Sofia 97, 95123 Catania (CT)

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