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Association of Thrombospondin-1 (N700S) and Thrombospond in-4 (A387P) Gene Polymorphisms with the Incidence of Acute Myocardial Infarction in Egyptians

[ Vol. 18 , Issue. 13 ]

Author(s):

Noura A. Abdelmonem, Nancy O. Turky, Ingy M. Hashad, Mohamed F. Abdel Rahman, Adel El-Etriby and Mohamed Z. Gad*   Pages 1078 - 1087 ( 10 )

Abstract:


Background: Thrombospondin (TSP) 1 and 4 are extracellular matrix glycoproteins that mediate cell proliferation, platelet aggregation and inflammatory response. Conflicting data addressed the possible contribution of TSP-1 and TSP-4 gene polymorphisms to acute myocardial infarction (AMI).

Objective: Our study aimed to examine the association of TSP-1 (N700S) and TSP-4 (A387P) genetic variants with the incidence of AMI in Egyptians. It also correlated TSP-1 variants to TSP-1 and TNF-α serum concentrations while TSP-4 variants to IL-8 concentration identifying TSPs' contribution to vascular inflammation.

Methods: Genotyping was done in 214 subjects; 114 AMI patients and 100 controls using PCR-RFLP analysis. Serum Tsp-1, TNF-α and IL-8 levels were measured by ELISA assay.

Results: For TSP-4, (GC and CC) genotype distribution and the (C) allele frequency were significantly higher in AMI patients than controls (p = 0.0186), (p = 0.0117) respectively. In contrast, TSP-1 genotypes and allele frequencies showed no significant difference between AMI and controls (p = 0.7124 and p = 0.7201, respectively). Serum TSP-1, TNF-α and IL-8 concentrations were significantly elevated in AMI compared to controls (p = 0.0146, p < 0.0001 and p = 0.0057) respectively. Serum IL-8 levels had a significant difference among TSP-4 genotypes (p= 0.0368), being highest in the mutant C allele. Serum TSP-1 and TNF-α concentrations showed no significant difference among TSP-1 genotypes, but there was a positive correlation between both concentrations in AMI patients (p = 0.0014), (r = 0.4125).

Conclusion: TSP-4 A387P polymorphism, but not TSP-1 polymorphism, is an independent risk factor for AMI in the Egyptians.

Keywords:

Acute myocardial infarction, thrombospondin, tumor necrosis factor alpha, interleukin-8, polymorphism, Egyptians, PCR-RFLP.

Affiliation:

Biochemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, Cairo, Biochemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, Cairo, Biochemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, Cairo, Biochemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, Cairo, International Cardioscan Center, Cairo, Biochemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, Cairo

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