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Determination and Isolation of Four Anti-tumour Saponins from Lonicera macranthoides by HPLC-ESI-QTOF/MS and HSCCC

[ Vol. 18 , Issue. 13 ]

Author(s):

Xuehui Liu, Rongrong Zhou, Bingbing Shen, Lin Chen, Qingyijun Zhou, Dan Wan, Luqi Huang* and Shuihan Zhang*   Pages 1106 - 1114 ( 9 )

Abstract:


Background: Lonicera macranthoides is a Chinese herb that contains a large number of bioactive spanions possessing important pharmacological activities, such as anti-tumour activity. However, detailed information about their anti-tumor activity and bioactive compounds is limited.

Methods: In order to evaluate the scientific basis, the method of high-speed counter-current chromatography (HSCCC) combined with high performance liquid chromatography mass spectrometry (HPLC-ESI-QTOF/MS) has been developed to separate, purify and analyze saponins from Lonicera macranthoides. Four main saponins, Macranthoidin B (I), Macranthoidin A (II), Macranthoides B (III) and Akebia saponin D (IV) were separated by HSCCC with the solvent systems of ethyl acetate-nbutanol- water (3:2:5) and n-butanol-water-methanol-ethyl acetate (1:6:0.5:4). The purities of these four bioactive ingredients (I-IV) identified and detected by HPLC-ESI-QTOF/MS were 95.1%, 92.7%, 91.8% and 96.3%, respectively. The separated saponins were evaluated for their cytotoxic activities against six tumor cell lines (MCF-7, Hela, A549, HepG2, HT29 and Eca109).

Results: Results show that compounds I-IV exhibited particular significant anti-tumor activities against human mammary adenocarcinoma MCF-7 cell with IC50 values ranging from 12.7 to 30.8 µM.

Conclusion: It was demonstrated that the combinative method using HPLC-ESI-QTOF/MS and HSCCC was suitable for rapid screening and isolating saponins of Lonicera macranthoides and the isolated compounds have great potential for the development of new antitumor drugs.

Keywords:

Lonicera macranthoides, HSCCC, saponins, HPLC-ESI-QTOF/MS, isolation, anti-tumor.

Affiliation:

Hunan Academy of Chinese Medicine, Research Institute of Chinese Medicine, Changsha, Changchun University of Chinese Medcine, College of Pharmacy, Changchun, Hunan Academy of Chinese Medicine, Research Institute of Chinese Medicine, Changsha, Hunan Academy of Chinese Medicine, Research Institute of Chinese Medicine, Changsha, Hunan Academy of Chinese Medicine, Research Institute of Chinese Medicine, Changsha, Hunan Academy of Chinese Medicine, Research Institute of Chinese Medicine, Changsha, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, Hunan Academy of Chinese Medicine, Research Institute of Chinese Medicine, Changsha

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