Uros Cakar*, Nada Grozdanic, Aleksandar Petrovic, Boris Pejin*, Branislav Nastasijevic, Bojan Markovic and Brizita Dordevic Pages 1264 - 1272 ( 9 )
Background: Fruit wines are well known for their profound health-promoting properties including both enzyme activations and inhibitions. They may act preventive in regard to diabetes melitus and other chronic diseases.
Objectives: Potential α-glucosidase inhibitory activity of fruit wines made from blueberry, black chokeberry, blackberry, raspberry and sour cherry was the subject of this study.
Method: In order to increase the alcohol content due to enriched extraction of total phenolics, sugar was added in the fruit pomace of the half of the examined fruit wine samples.
Results: Compared with acarbose used as a positive control (IC50 = 73.78 µg/mL), all fruit wine samples exhibited higher α-glucosidase inhibitory activity. Indeed, blueberry wine samples stood out, both prepared with IC50 = 24.14 µg/mL, lyophilised extract yield 3.23% and without IC50 = 46.39 µg/mL, lyophilised extract yield 2.89% and with addition of sugar before fermentation. Chlorogenic acid predominantly contributed to α-glucosidase inhibitory activity of the blueberry, black chokeberry and sour cherry wine samples. However, ellagic acid, a potent α-glucosidase inhibitor possessing a planar structure, only slightly affected the activity of the blueberry wine samples, due to the lower concentration. In addition to this, molecular docking study of chlorogenic acid pointed out the importance of binding energy (-8.5 kcal/mol) for the inhibition of the enzyme.
Conclusion: In summary, fruit wines made from blueberry should be primarily taken into consideration as a medicinal food targeting diabetes mellitus type 2 in the early stage, if additional studies would confirm their therapeutic potential for the control of postprandial hyperglycemia.
Fruit wines, blueberry, α-glucosidase inhibitory activity, chlorogenic acid, molecular docking, medicinal food.
University of Belgrade,Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Institute for Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080, Belgrade-Zemun, University of Belgrade, Institute for Multidisciplinary Research-IMSI, Kneza Viseslava 1, 11030, Belgrade, University of Belgrade, Vinca Institute of Nuclear Sciences, P. Box 552, 11001 Belgrade, University of Belgrade,Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, University of Belgrade,Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade