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High Level of Menaquinone-7 Production by Milking Menaquinone-7 with Biocompatible Organic Solvents

[ Vol. 19 , Issue. 3 ]

Author(s):

Dinali Ranmadugala, Alireza Ebrahiminezhad*, Merilyn Manley-Harris, Younes Ghasemi and Aydin Berenjian*   Pages 232 - 239 ( 8 )

Abstract:


Background: The effect of milking menaquinone-7 (MK-7) with biocompatible organic solvents on MK-7 production in B. subtilis fermentation was studied. Periodic milking of MK-7 from the fermentation medium by n-hexane significantly enhanced the total MK-7 production ~ 1.7 fold at the end of 72 hours of fermentation (p < 0.05) as compared to the control medium.

Methods: Milking of MK-7 with a mixture of n-hexane phase modified with n-butanol was also explored. Although milking of MK-7 by a mixture of n-hexane and n-butanol (1:2, v/v), was found to be appropriate in terms of high extraction capacity, no significant increase in total MK-7 concentration was observed. Biocompatibility between the extraction solvents and B. subtilis was also examined.

Results and Conclusion: The results indicated that the mixture of n-hexane and n-butanol exhibited some detrimental effects. However, n-hexane alone exhibited delayed toxicity starting at 84 hours of periodic milking and could, therefore, be considered as the most promising organic solvent for milking MK-7 in B. subtilis fermentation while enhancing the productivity of the system.

Keywords:

MK-7, B. subtilis, milking, fermentation, viability, n-hexane, n-butanol.

Affiliation:

Faculty of Science and Engineering, The University of Waikato, Hamilton, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Faculty of Science and Engineering, The University of Waikato, Hamilton, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Faculty of Science and Engineering, The University of Waikato, Hamilton

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