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Multi-walled Carbon Nanotubes Reinforced into Hollow Fiber by Chitosan Sol-gel for Solid/Liquid Phase Microextraction of NSAIDs from Urine Prior to HPLC-DAD Analysis

[ Vol. 20 , Issue. 5 ]

Author(s):

Nabil N. AL-Hashimi*, Amjad H. El-Sheikh, Rania F. Qawariq, Majed H. Shtaiwi and Rowan AlEjielat   Pages 390 - 400 ( 11 )

Abstract:


Background: The efficient analytical method for the analysis of nonsteroidal antiinflammatory drugs (NSAIDs) in a biological fluid is important for determining the toxicological aspects of such long-term used therapies.

Methods: In the present work, multi-walled carbon nanotubes reinforced into a hollow fiber by chitosan sol-gel assisted-solid/ liquid phase microextraction (MWCNTs-HF-CA-SPME) method followed by the high-performance liquid chromatography-diode array detection (HPLC–DAD) were developed for the determination of three NSAIDs, ketoprofen, diclofenac, and ibuprofen in human urine samples. MWCNTs with various dimensions were characterized by various analytical techniques. The extraction device was prepared by immobilizing the MWCNTs in the pores of 2.5 cm microtube via chitosan sol-gel assisted technology while the lumen of the microtube was filled with few microliters of 1-octanol with two ends sealed. The extraction device was operated by direct immersion in the sample solution.

Results: The main factors influencing the extraction efficiency of the selected NSAIDs have been examined. The method showed good linearity R2 ≥ 0.997 with RSDs from 1.1 to 12.3%. The limit of detection (LOD) was 2.633, 2.035 and 2.386 μg L-1, for ketoprofen, diclofenac, and ibuprofen, respectively. The developed method demonstrated a satisfactory result for the determination of selected drugs in patient urine samples and comparable results against reference methods.

Conclusion: The method is simple, sensitive and can be considered as an alternative for clinical laboratory analysis of selected drugs.

Keywords:

Multi-walled carbon nanotubes, reinforced-solid/liquid microextraction, Chitosan sol-gel, NSAIDs, HPLC-DAD, urine.

Affiliation:

The Hashemite University, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, P.O. Box 330127, Al-Zarqa 13133, The Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa 13115, The Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa 13115, The Hashemite University, Faculty of Science, Department of Chemistry, P.O. Box 150459, Al-Zarqa 13115, Amarican University of Madaba, Faculty of Health Science, Department of Pharmacy, P.O. Box 2882, Amman



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