Gusti Sartono, Izzatu Rizqiyah, Asmarinah, Nicholas C.K. Heng and Amarila Malik* Pages 766 - 771 ( 6 )
Background: The development of antibiotic resistance amongst bacterial pathogens and a population explosion, e.g. in countries such as Indonesia, are two issues the world is facing today. These issues have stimulated interest in the development of new antimicrobial therapeutic agents and contraceptive strategies, such as novel spermicides. Bacteriocins, which are bacterially-derived antimicrobial peptides, may fulfill some of the criteria for these new agents.
Methods: Weissella confusa MBF8-1, originally isolated from a homemade soy product, exhibits antibacterial activity that was subsequently found to be plasmid-encoded, presumably by three peptides Bac1, Bac2 and Bac3. In the present study, we tested cell-free MBF8-1 bacteriocin preparations and chemically-synthesized versions of Bac1, Bac2 and Bac3 peptides for (i) its antibacterial activity against the indicator bacterium Leuconostoc mesenteroides and (ii) its ability to affect the motility of spermatozoa. Nisin, a known lantibiotic bacteriocin, was used as the control.
Results: Here, we demonstrate that synthetic Bac1, in combination with synthetic Bac2, was sufficient to inhibit the growth of L. mesenteroides and affect sperm motility. However, the presence of all three synthetic peptides, s-Bac1, s-Bac2 and s-Bac3, was required for full potency.
Conclusion: In summary, the bacteriocin-like peptides of W. confusa MBF8-1 have the potential to be developed as a narrow-spectrum antimicrobial agent and a novel spermicidal agent.
Antibacterial activity, bacteriocin, Weissela confusa, weissellicin MBF, Sander-Cramer, spermicidal activity.
Pharmaceutical Microbiology and Biotechnology Division, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, Pharmaceutical Microbiology and Biotechnology Division, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424, Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, UI Salemba Campus, Jalan Salemba Raya no. 6, Jakarta 10430, Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, Pharmaceutical Microbiology and Biotechnology Division, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok 16424