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The Ultrashort Peptide OW: A New Antibiotic Adjuvant

[ Vol. 20 , Issue. 9 ]

Author(s):

Yara Al Tall*, Ahmad Abualhaijaa, Mohammed T. Qaoud, Mohammad Alsaggar, Majed Masadeh and Karem H. Alzoubi   Pages 745 - 754 ( 10 )

Abstract:


Background: The overuse of the current antibiotics and the low discovery rate of new ones are leading to rapid development of multidrug-resistant pathogens worldwide. Antimicrobial Peptides has shown promising results against multidrug-resistant bacteria.

Objective: To investigate the antimicrobial activity of a new ultrashort hexapeptide (OW).

Methods: The OW hexapeptide was designed and tested against different strains of bacteria with different levels of sensitivity. Bacterial susceptibility assays were performed according to the guidelines of the Clinical and Laboratory Institute (CLSI). The synergistic studies were then tested using Checkerboard assay. This was followed by checking the hemolytic effect of the hexapeptide against human blood cells and Human Embryonic Kidney cell line (HEK293). Finally, the antibiofilm activities of the hexapeptide were studied using Biofilm Calgary method.

Results: The synergistic assays showed that OW has synergistic effects with antibiotics of different mechanisms of action. It showed an outstanding synergism with Rifampicin against methicillin resistant Staphylococcus aureus; ΣFIC value was 0.37, and the MIC value of Rifampicin was decreased by 85%. OW peptide also displayed an excellent synergism with Ampicillin against multidrug-resistant Pseudomonas aeruginosa, with ΣFIC value of less than 0.38 and a reduction of more than 96% in the MIC value of the Ampicillin.

Conclusion: This study introduces a new ultrashort peptide (OW) with promising antimicrobial potential in management of drug-resistant infectious diseases as a single agent or in combination with commonly used antibiotics. Further studies are needed to investigate the exact mechanism of action for these peptides.

Keywords:

Antimicrobial resistance, ultrashort peptide, antibiotic adjuvant, synergism, MIC, hemolytic assay.

Affiliation:

Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid



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