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Changes in the Expression Profile of VEGF-A, VEGF-B, VEGFR-1, VEGFR-2 in Different Grades of Endometrial Cancer

[ Vol. 20 , Issue. 11 ]

Author(s):

Konrad Dziobek*, Marcin Opławski, Beniamin Oskar Grabarek, Nikola Zmarzły, Barbara Tomala, Tomasz Halski, Ewa Leśniak, Krzysztof Januszyk, Ryszard Brus, Robert Kiełbasiński and Dariusz Boroń   Pages 955 - 963 ( 9 )

Abstract:


Background: VEGF-A, VEGF-B, VEGFR-1 and VEGFR-2 are important proteins involved in the induction and development of a new blood vessel network through which the tumor is properly nourished and oxygenated.

Objectives: The aim of the study was to evaluate changes in VEGF-A, VEGF-B, VEGFR-1 and VEGFR-2 expression in endometrial cancer depending on its grade and to determine the VEGFR-1 to VEGFR-2 concentration ratio.

Methods: The study group consisted of 45 patients diagnosed with endometrial cancer (G1, 17; G2, 15; G3, 13). The control group included 15 patients. VEGF-A, VEGF-B, VEGF-R1, VEGFR-2 expression was assessed using the immunohistochemical method. Statistical analysis was carried out using the Statistica 12 PL program (StatSoft, Cracow, Poland). It included the one-way ANOVA and Tukey's post-hoc test (p<0.05).

Results: Statistically significant differences in the level of VEGF-A, VEGF-B, VEGF-R1, VEGFR-2 were observed between the majority of analyzed groups (except for VEGF-B; G3 vs. G1, p=0.997700). The expression pattern of VEGF-A, VEGF-R1, VEGFR-2 was as follows: G3>G2>G1>C; VEGF-B: G2> G3> G1>C. A lower concentration of VEGFR-1 than VEGFR-2 was found regardless of the cancer grade.

Conclusion: VEGF-A, VEGF-B, VEGF-R1, VEGFR-2 are key proteins involved in tumor angiogenesis. The analysis of the entire panel of proteins participating in a given process is an important element of modern diagnostics. The concentration ratio of VEGFR-1 to VEGFR-2 appears to be a determining factor in the patients' survival prognosis.

Keywords:

VEGF-A/B, VEGFR-1/2, angiogenesis, endometrial cancer, tumor angiogenesis, adenomyosis.

Affiliation:

Center of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Branch, Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, Kraków, Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Faculty of Health Science, Public Higher Medical Professional School in Opole, Faculty of Health Science, Public Higher Medical Professional School in Opole, Faculty of Health Science, Public Higher Medical Professional School in Opole, Faculty of Health Science, Public Higher Medical Professional School in Opole, Department of Nurse, High School of Strategic Planning, Koscielna 6, 41-303, Dabrowa Gornicza, Department of Obstetrics & Gynaecology ward, Health Center in Mikołów, Mikołów, Center of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Branch



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