Ahmed M. Younis*, Marwa M. Abdel-Aziz and Mohamed Yosri* Pages 1309 - 1320 ( 12 )
Background: Mushrooms are deemed as a special delicacy in many countries. They are considered an important cuisine due to their bioactive ingredients and possible health benefits.Methods: Herein, we measured selected biological properties of methanol extracts of Pleurotus citrinopileatus and Boletus edulis fruiting bodies including; in vitro antimicrobial activity, anti-α- glucosidase activity, antioxidant activity, anti-lipase activity and cytotoxic activity against different cancer cells and normal cells. Results: B. edulis methanol extracts showed high antimicrobial and anti-α-glucosidase activity. In contrast, P. citrinopileatus methanol extracts showed superior antioxidant activity indicated by (1,1- diphenyl-2-picrylhydrazyl) DPPH radical scavenging with half maximal inhibitory concentration of IC50 37.4 μg/ml, anti-lipase activities with IC50 65.2 μg/ml and high cytotoxicity activity against HepG2 and HeLa cell lines with IC50 22.8 and 36.7 μg/ml, respectively. Flow cytometric analysis of the cell cycle was used to show apoptotic effects of methanol extracts against HepG2 and HeLa cells. Conclusion: P. citrinopileatus and B. edulis methanolic extracts appear to contain biologically active compounds that might be used to treat some common human diseases.
Pleurotus citrinopileatus, Boletus edulis, antimicrobial activity, antioxidant activity, antitumor activity, anti-α- glucosidase activity, anti-lipase activity, apoptosis.
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, The Regional Center for Mycology and Biotechnology, Al Azhar University, 11787 Nasr City, Cairo, The Regional Center for Mycology and Biotechnology, Al Azhar University, 11787 Nasr City, Cairo