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Variances in the Level of COX-2 and iNOS in Different Grades of Endometrial Cancer

[ Vol. 21 , Issue. 1 ]

Author(s):

Marcin Oplawski*, Konrad Dziobek, Nikola Zmarzły, Beniamin O. Grabarek, Robert Kiełbasiński, Przemysław Kieszkowski, Piotr Januszyk, Karol Talkowski, Michał Schweizer, Piotr Kras, Andrzej Plewka and Dariusz Boroń   Pages 52 - 59 ( 8 )

Abstract:


Background: Many experimental studies have demonstrated the importance of COX-2 in the tumor angiogenesis. Inducible iNOS is responsible for a high and stable level of nitric oxide and is expressed in response to pro-inflammatory factors.

Objective: The aim of this study was to evaluate the expression of COX-2 and iNOS at the protein level and to assess their potential prognostic significance in patients with endometrial cancer.

Methods: The study group consisted of 45 women with endometrial cancer divided according to the degree of histological differentiation i.e. G1, 17; G2, 15; G3, 13. The control group consisted of 15 women without neoplastic changes. The expression of studied proteins was determined immunohistochemically with specific polyclonal antibodies.

Results: Analysis of the COX-2 expression showed that the optical density of the reaction product in G1 reached 186% in the control group, while the values in G2 and G3 reached 243% and 293%, respectively. In the case of iNOS, the optical density of the reaction product reached the following percentages in the control group: 147% in G1, 243% in G2, and 241% in G3.

Conclusion: Our findings suggest that changes in the expression of COX-2 and iNOS may be potentially useful in predicting the progression of endometrial cancer and treatment effectiveness.

Keywords:

Endometrial cancer, angiogenesis, COX-2, iNOS, neoplastic changes, molecular marker.

Affiliation:

Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, Kraków, Center of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Branch, Cracow, Katowice School of Technology, The University of Science and Art in Katowice, Katowice, Katowice School of Technology, The University of Science and Art in Katowice, Katowice, Department of Obstetrics & Gynaecology Ward, Health Center in Mikołów, Mikołów, Voivodeship Specialist Hospital in Wloclawek, Wloclawek, Katowice School of Technology, The University of Science and Art in Katowice, Katowice, University of Warmia and Mazury in Olsztyn, Department of Psychiatry, Olsztyn, Center of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Branch, Cracow, Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, Kraków, Faculty of Health Science, Public Higher Medical Professional School in Opole, Opole, Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, Kraków

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