Fatma Kazdal, Fatemeh Bahadori*, Burak Celik, Abdulselam Ertas and Gulacti Topcu Pages 681 - 701 ( 21 )
Background: The role of Fe+2, Cu+2 and Zn+2 in facilitating aggregation of Amyloid β (Aβ) and consequently, the progression of Alzheimer's disease (AD) is well established.Objective: Development of non-toxic metal chelators is an emerging era in the treatment of AD, in which complete success has not been fully achieved. The purpose of this study was to determine plant extracts with high metal chelator and to encapsulate them in nano-micellar systems with the ability to pass through the Blood Brain Barrier (BBB). Methods: Extracts of 36 different Anatolian plants were prepared, total phenolic and flavonoid contents were determined, and the extracts with high content were examined for their Fe+2, Cu+2 and Zn+2 chelating activities. Apolipoprotein E4 (Apo E) decorated nano-formulations of active extracts were prepared using Poly (Lactide-co-Glycolide) (PLGA) (final product ApoEPLGA) to provide BBB penetrating property. Results: Verbascum flavidum aqueous extract was found as the most active sample, incubation of which, with Aβ before and after metal-induced aggregation, resulted in successful inhibition of aggregate formation, while re-solubilization of pre-formed aggregates was not effectively achieved. The same results were obtained using ApoEPLGA. Conclusion: An optimized metal chelator nano-formulation with BBB penetrating ability was prepared and presented for further in-vivo studies.
Alzheimer, apo E4, metal chelator, nano-drug delivery, polyphenol, amyloid β.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Bezmialem Vakif University, 34093, Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Bezmialem Vakif University, 34093, Department of Pharmaceutical Technology, Faculty of Pharmacy, Bezmialem Vakif University, 34093, Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Department of Pharmacognosy, Faculty of Pharmacy, Bezmialem Vakif University, 34093