Konrad Dziobek*, Marcin Oplawski, Nikola Zmarzły, Beniamin O. Gabarek, Robert Kiełbasiński, Kamil Kiełbasiński, Przemysław Kieszkowski, Karol Talkowski and Dariusz Boroń Pages 635 - 641 ( 7 )
Background: Endometrial cancer is one of the most common gynecological cancer in the developed countries and occurs mainly in postmenopausal women. Angiogenesis is important for cancer formation as it provides nutrients for growing tumor mass. Most tumors do not show detectable Homeobox A5 (HOXA5 level), suggesting its potential role as a cancer suppressor. It was demonstrated that HOXA5 is involved in the progression of various types of cancer and the loss of its expression correlates with higher pathological grade and poorer outcome.
Objective: The aim of the study was to evaluate HOXA5 expression at transcriptome and protein levels.
Materials and Methods: The study enrolled 45 women diagnosed with endometrial cancer and 15 without neoplastic changes. The histopathological examination allowed us to divide cancer tissue samples according to the degree of histological differentiation: G1, 17; G2, 15; G3, 13. The expression of the HOXA5 protein was determined by immunohistochemistry. Microarray and RT-qPCR techniques were used to assess HOXA5 expression at the mRNA level.
Results: The reaction to the HOXA5 protein was only visible in glandular cells in G1 endometrial cancer and was lower compared to the control. In grades 2 and 3, reactions were noted at the limit of the method’s sensitivity. In addition, reduced HOXA5 expression was observed at the transcriptome level.
Conclusion: HOXA5 may become a potential complementary molecular marker, allowing early detection of neoplastic changes in the endometrium. It also seems that detection of HOXA5 at the mRNA and protein levels may be helpful in improving the accuracy of diagnosis and planning effective oncological therapy.
Endometrial cancer, HOXA5, immunohistochemistry, RT-qPCR, microarray, mRNA level.
Maria Sklodowska-Curie National Research Institute of Oncology Krakow Branch, 11 Garncarska st. 31-115 Krakow, Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, Krakow, Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology in Katowice, Zabrze, Maria Sklodowska-Curie National Research Institute of Oncology Krakow Branch, 11 Garncarska st. 31-115 Krakow, Department of Obstetrics & Gynaecology Ward, Health Center in Mikolow, Mikolow, Department of Obsterics and Gynecology in Ruda Slaska, Medical University of Silesia, Ruda Slaska, Voivodeship Specialist Hospital in Wloclawek, Wloclawek, Psychiatric Hospital in Olsztyn, Olsztyn, Maria Sklodowska-Curie National Research Institute of Oncology Krakow Branch, 11 Garncarska st. 31-115 Krakow