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Screening of an Alkaline CMCase-Producing Strain and the Optimization of Its Fermentation Condition

[ Vol. 21 , Issue. 13 ]

Author(s):

Junmei Zhou, Lianghong Yin*, Chenbin Wu, Sijia Wu, Jidong Lu, Hailing Fang and Yongchang Qian*   Pages 1304 - 1315 ( 12 )

Abstract:


Objective: Alkaline carboxymethyl cellulase (CMCase) is an attractive enzyme for the textile, laundry, pulp, and paper industries; however, commercial preparations with sufficient activity at alkaline conditions are scarce.

Methods: High CMCase-producing bacterial isolate, SX9-4, was screened out from soil bacteria, which was identified as Flavobacterium sp. on the basis of 16S rDNA sequencing.

Results: The optimum pH and temperature for CMCase reaction were 8.0 and 55oC, respectively. Alkaline CMCase was stable over wide pH (3.0-10.6) and temperature (25-55oC) ranges. Enzyme activity was significantly inhibited by the bivalent cations Mn2+ and Cu2+, and was activated by Fe2+. To improve the alkaline CMCase production of SX9-4, fermentation parameters were selected through onefactor- at-a-time and further carried out by response surface methodologies based on a central composite design.

Conclusion: High CMCase production (57.18 U/mL) was achieved under the optimal conditions: 10.53 g/L carboxymethylcellulose sodium, 7.74 g/L glucose, 13.71 g/L peptone, and 5.27 g/L ammonium oxalate.

Keywords:

Alkaline CMCase, screening, identification, enzyme characteristics, optimization, fermentation.

Affiliation:

School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300



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