Submit Manuscript  

Article Details


MiR-493 Induces Cytotoxic Autophagy in Prostate Cancer Cells through Regulation on PHLPP2

[ Vol. 21 , Issue. 14 ]

Author(s):

Jun Deng, Ming Ma , Wei Jiang, Liangliang Zheng and Suping Cui *   Pages 1451 - 1456 ( 6 )

Abstract:


Background: MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting PHLPP2. We aimed to explore the relationship between miR-493 and autophagy in PC.

Methods: qRT-PCR and western blotting were used to determine the mRNA levels and protein expression of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding changes of autophagy phenotype and PC cell function were also studied.

Results: The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC cells were significantly decreased in PC cells. Overexpression of miR-493 or PHLPP2 markedly upregulated the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells.

Conclusion: MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition by regulating on PHLPP2.

Keywords:

MiR-493, Prostate cancer, PHLPP2, autophagy, qRT-PCR, PC cells.

Affiliation:

Department of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Department of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Department of Stomatology, Nanchang Second Hospital, Nanchang 330006, Department of Urology, Graduate School of Nanchang University, Nanchang 330006, Department of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006



Read Full-Text article