Li Xiaobing, Niu Chunling, Chen Wenyu, Chen Yan and Li Zhenzhen* Pages 1204 - 1212 ( 9 )
Objectives: To investigate the effect of Danggui-Shaoyao-San (DSS)-containing serum on the renal tubular Epithelial-Mesenchymal Transition (EMT) of Diabetic Nephropathy (DN) in high glucose- induced HK-2 cells and its mechanism.
Methods: 20 rats were randomly divided into four groups: blank control group, DSS low dose group (DSS-L), DSS middle dose group (DSS-M), and DSS high dose group (DSS-H). DSS was administrated to the corresponding group (7g/kg/d, 14g/kg/d and 21g/kg/d) for 7 consecutive days, and the same volume of saline was given to the blank control group by gavage. The rat drug-containing serum was successfully prepared. HK-2 cells were divided into five groups: blank control group, model group, DSS-L, DSS-M, DSS-H, according to the corresponding drug and dose of each treatment group. Protein and mRNA levels of Jagged1, Notch1, Hes5, Notch Intracellular Domain (NICD), E-cadherin, alpha- Smooth Muscle Actin (α-SMA) and vimentin at 24h, 48h and 72h were detected by Western Blot and RT-qPCR.
Results: The protein and mRNA levels of Jagged1, Notch1, Hes5, NICD, α-SMA and vimentin in the treatment groups were remarkably decreased compared with the model group (P<0.05), and the protein and mRNA levels of E-cadherin were notably increased (P<0.05) by Western Blot and RT-qPCR.
Conclusion: Our results demonstrated that DSS could prevent DN by ameliorating renal tubular EMT through inhibition of the Notch signaling pathway.
Diabetic nephropathy, Notch signalling pathway, Renal tubular epithelial-mesenchymal transition, Danggui- Shaoyao-San-containing serum, Renal fibrosis, diabetes mellitus.
College of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450000, College of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450000, The Second School of Clinical Medicine of Henan University of Traditional Chinese Medicine, Zhengzhou 450011, The Second School of Clinical Medicine of Henan University of Traditional Chinese Medicine, Zhengzhou 450011, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000