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Leaf and Fruit Methanolic Extracts of Azadirachta indica Exhibit Antifertility Activity on Rats’ Sperm Quality and Testicular Histology

Author(s):

Irais Castillo-Maldonado, Claudia Borjón-Ríos, David Pedroza-Escobar, Ashutosh Sharma, Rubén García-Garza, Agustina Ramírez-Moreno, María del CarmenVega-Menchaca, Mario Alberto Rivera-Guillén and Luis-Benjamín Serrano-Gallardo*   Pages 1 - 8 ( 8 )

Abstract:


Background: The world's population is still growing, having an impact on the environment and the economic growth of developing countries; so that, there is currently a particular interest in the development of new fertility control methods, focused on male contraception. The objective of this study was to evaluate the effect of methanolic extracts of leaf and fruit of Azadirachta indica on sperm quality and testicular histology of Long Evans rats.

Method: Antifertility effects of a methanolic leaf and fruit extracts of A. indica on 24 male rats were investigated. The animals were randomly divided into two control groups and four treatment groups (n=4). Doses of the leaf and fruit extract were given at concentrations of 100 and 200 µg mL-1.

Results: A significant decrease in the viability of sperm cells was observed. The leaf extract at a concentration of 200 µg mL-1 inhibited cell viability compared to the negative control (p˂ 0.001). The percentage of abnormal cells is shown being leaf extract at 100 and 200 µg mL-1, the conditions at which a higher percentage of morphological irregularities were observed (15% and 16% respectively). The results show that there was cellular detachment in the seminiferous epithelium in the experimental groups treated with methanolic extracts. Sperm death was observed without decreasing the number of sperm.

Conclusion: The methanolic extracts of Azadirachta indica have a modulating effect on the spermatogenesis of experimental rats through sperm morphological alterations.

Keywords:

Antifertility, methanolic extract, sperm count, morphological characteristics, sperm motility, Azadirachta indica.

Affiliation:

Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Department of Bioengineering, School of Engineering and Sciences, Tecnologico de Monterrey, Centre of Bioengineering, Campus Queretaro, Queretaro, Department of Histology, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Faculty of Biological Sciences, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Faculty of Chemical Sciences, Universidad Juarez del Estado de Durango, Gomez Palacio, Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon



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