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Development and Characteristics of Novel PLGA-chitosan as a New Nano-carrier for Pentavalent Vaccine Delivery


Zahra Alikhani, Nadia Kazemi-Pour*, Mehdi Shafieeardestani* and Mojtaba Noofeli   Pages 1 - 13 ( 13 )


Background: Lately, the employment of nano-carriers has been known as an optimistic means of drug and vaccine delivery.

Objective: Nano vaccines are a novel tier of vaccines that can develop humoral and cellular immune responses and can be introduced as a practical and secure nano vaccine candidate the prevention of diseases. The purpose of this study was to accomplish and use biodegradable nano-carriers for the synthesis of pentavalent vaccine and immunogenicity evaluation in the animal models. The PLGA nanoparticle was prepared and modified with chitosan nanoparticle. Nano-carrier PLGA-chitosan was loaded by the DTP-HepB-Hib antigens and confirmed by DLS, SEM, TEM and FT-IR then the in vitro loading and release were evaluated. The toxicity was assessed by the MTT method in the Hec293 cells. The antigenicity evaluation and histopathological study were conducted by injection of new nano pentavalent vaccines in the BALB/c mice and the immune response was measured in the serum samples through an indirect ELISA method.

Results: Conclusions drawn from the current study exhibited the plausible ability of nano-carrier to deliver vaccine. A notable increase was shown in total IgG and IgM antibodies examined on mice vaccinated with new nano vaccines. The histopathological study in the treated mice showed no toxicity in vital organs of mice.

Conclusion: The engineered vaccine delivery system showed the ability to induce robust immune responses and also the suitability features of the PLGA-chitosan as a promising carrier to improve vaccine delivery.


Biodegradable; chitosan; PLGA; Pentavalent vaccine; Nano pentavalent vaccine.


Department of Microbiology, Kerman Islamic Azad University, Kerman, Department of Microbiology, Kerman Islamic Azad University, Kerman, Radio Pharmacy Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Human Bacterial Vaccines Production & Research, Razi Vaccine & Serum Research Institute (RVSRI), Karaj; Agricultural Research, Education & Extension Organization (AREEO), Tehran

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