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Effect of TRAF1 on Phenotypic Changes of Kupffer Cells in Liver Transplantation Ischemic-Reperfusion

Author(s):

Zhen Zhang, KeTing Que, Yu You, XiaoPing Zhao, DiGuang Wen, ZhiHao Feng and ZuoJin Liu*   Pages 1 - 10 ( 10 )

Abstract:


Objective: The purpose of this study was to explore the effect of TRAF1 on phenotypic changes of KCs in I/R in liver transplantation.

Methods: SD rats were randomly divided into sham group and liver transplantation I/R group. KCs were extracted from rat livers in each group. KCs were transfected by lentivirus of si-TRAF1 or si-HOIP, and induced by lipopolysaccharide (LPS). Flow cytometry was used to detect cell apoptosis. Western blot and RT-PCR were used to detect the protein and mRNA expression levels.

Results: Compared with sham group, the expression levels of TRAF1, TNF-α and IL-1β were significantly increased in I/R group (P<0.05). In addition, compared with control group, the expression levels of NF-κB (P65 and p-P65) and M1 phenotype (TNF-α and IL-1β) were notably increased in si-TRAF1 and si-HOIP group (all P<0.05). Furthermore, the levels of linear ubiquitin complex (LUBAC) were markedly increased in LPS-induced KCs in comparison with control group (P<0.05). Moreover, compared with control group, the expression levels of P65, p-P65, TNF-α and IL-1β were notably decreased in si-TRAF1 and si-HOIP group (P<0.05). The expression levels of P65, p-P65, TNF-α and IL-1β were significantly increased in si-TRAF1 and si-HOIP group when compared to control group (P<0.05).

Conclusion: TRAF1 was an important negative regulator of liver transplantation I/R by inhibiting the activation of NF-κB in KCs and preventing its M1 phenotype transformation.

Keywords:

Kupffer cells, TRAF1; NF-κB, liver transplantation, ischemia reperfusion, M1 phenotype, lipopolysaccharide

Affiliation:

Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010



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