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Optimal Enzymes for Single-Molecule Sequencing

[ Vol. 5 , Issue. 1 ]

Author(s):

Susanne Brakmann   Pages 119 - 126 ( 8 )

Abstract:


Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemists point of view.

Keywords:

dna sequencing, single-molecule detection, dna polymerase, exonuclease, fluorescence

Affiliation:

Applied Molecular Evolution, Institute for Zoology, University of Leipzig, Liebigstrasse 18, D-04103Leipzig, Germany.



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